AFLP(Amplified fragment length polymorphism)is a newly developed molecular marker technique.Its basic principle was briefly described.The application of AFLP in Germ plasm resources,Genetics and Breeding of fruit trees in recent years were introduced.And the probles and practical prospects of AFLP technique were also evaluated.
【基金】
国家自然科学基金项目资助(30560090)
【更新日期】
2006-10-12
【分类号】
S66
【正文快照】
1 AFLP标记的基本原理AFLP(Amplified fragment length polymorphism,扩增片段长度多态性)的原理是基于对植物基因组总DNA双酶切经PCR扩增后的限制片段进行选择[1]。具体是植物基因组DNA经限制性内切酶双酶切后,形成分子量大小不等的随机限制性片段,将特定双链接头连接在这些DN