In this paper the extraction and the preliminary determination of flavonoids in Cucurbita moschata(Duch.) Poiret stem,leaf and flower were reported.The result indicated that the flavonoids content in Cucurbita moschata(Duch.) Poiret flower was highes:3.04%,leaf and burgeoning stem was:2.51% and 1.36%.It provided a theoretical basis for vtilization,production and process and utilization in Cucurbita moschata(Duch.) Poiret stem,leaf and flower.