There are three difficult problems in plant tissue culture: contamination,browning and vitrification. These badly affect the construction of plant culture and rapid propagation in vitro system. This article not only explains why these phenomena come out,but also poses some suggestions on how to prevent and control them.
【更新日期】
2006-11-14
【分类号】
Q943.1
【正文快照】
自20世纪初德国植物生理学家H abedandt提出植物细胞全能性理论以来,植物组织培养技术发展迅速。植物组织培养是在无菌条件下,将植物的离体器官、组织、细胞以及去除细胞壁的原生质体,经过人为控制环境条件,在合成培养基上对植物进行克隆,使其生长、分化并成长为完整植株。M ur