A study on the technique of tissue culture and plantlet regeneration of Fraxinus velutina stem was conducted.The results showed that WPM medium was its basic one and that media for the bud induction from stem segments and the rooting from shooting were WPM + TDZ 1.0 mg/L+ NAA(4.0) mg/L and WPM + IBA 1.0 mg/L + NAA 0.5 mg/L respectively.These results might provide references to the cell engineering and gene engineering of Fraxinus velutina.