Fuji,Nagafu2,Nagafu6,Starking,New Century,America8and Ryoka apple varieties were used as materials for ISSR fingerprinting,and their fingerprint were established.Results showed that primer IS061could distinguish one from the other among Fuji,Nagafu2and Nagafu6varieties;while Fuji,Starking,New Century and America8could be discriminat-ed by IS026。The results proved that apple cultivars could be distinguished by ISSR marker.