In this paper, the method of protein removal in the course of extracting cordyceps militaris is described. There are a lot of protein in extracting expolysaccharide and inpolysaccharide, Ration and removing protein are important problems. Based on orthogonal experiment, we find the optimum condition for remving protein from cordyceps polysaccharide: matching of exponent/chloroform butanol, 1∶24; volume matching of chloroform butanol,is 1∶0.2; abstracting time, 5?min. Polysaccharide rate obtained is 50%, ...