First strand cDNA was synthesized by reverse transcriptase using total RNA extracted from the midgut of Helicoverpa armigera larvae. With the first strand cDNA as template, and oligonucleotides designated on the conserved region in the trypsin like protease of insects and the reported sequence of Helicoverpa armigera as primers, a cDNA sequence encoding a trypsin like proteinase was amplified by RT PCR. The PCR products were cloned using the plasmid pBlueKS(+) as the vector. Insertion of the re...
【基金】
中国科学院特别支持费项目 (No STZ98 3 0 8);
国家“863”高技术计划基金 (No 2 0 0 1AA2 12 0 41和No 2 0 0 1AA2 2 2 2 5 1);
国家自然科学基金项目 (No 3 99890 0 1、No 3 9980 0 2 4和No 3 9880 0 2 3 );
国家转基因植物研究与产业化开发专题基金 (No J99 B 0
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