In this paper, we studied the fermentation conditions of Producing GLA by a mutant strain Mortierella sp.A02. It was cultured in shaking flash with different carbon source,nitrogen source temperature and different C/N ratio. The optimal carbon source is glucose and the optimal nitrogen source is yeast extract ,The optimal C/N ratio for lipid accumulating is 60∶1.The optimal temperature range is 25℃~30℃.The temperature rang 15℃~20℃is optimal for both lipid accumulation and GLA synthesizing.The collection rat...