This paper reports the studies on in vitro culture of shoot tip of tillered onion,including callus induction and differentiation,plantlet root development and transplantation.Results showed:(1)MS basal medium supplemen ted with 2,4 D 2.0mg·L -1 and KT 0.5mg·L -1 was advantageous to callus induction.The frequency of callus induction was 100%.(2)The combination of NAA 0.1mg·L -1 and BA 0.4mg·L -1 was the best for callus differentiation with the ratio of 88.3%.Most of callus was agg...