The techniques of separation of anthocyan from grape seeds were studied.The grape seeds after being soaked in cold methyl alcohol for 12 h were extracted under reflux 3 times (2 h each time) at 70 ℃.The raffinate was concentrated under vacuum into extract,which was then dissolved in boiling water and filtered.The filtrate was extracted 2 times by n butanol.The extract was concentrated under vacuum,and then dried under vacuum to give the final product anthocyan.Purity of anthocyan was above 9...
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