The solid fermentation condition of Aspergillus niger SL2-111 was studied by using single-factor research and orthogonal test based on the response value of acid protease. The results showed that the optimal medium contained 8.25 g fresh bran, 4.5 g rice bran, 1.5 g cake bean, 0.3 g (NH4)2SO4, 0.06 g K2HPO4, 0.075 g CaCl2, 8.6 mL water. The initial pH was at 5.5, and culture temperature was fermented at 28℃ for 30 h and then at 23℃ for later 30 h. The enzyme activities of acid protease, pectinase and cellu...
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