Different malt soaking degree,Na 2SeO 3 and malting additive concentration on barley Se- enrichment were determined.The results showed that the best condition was 42% malt soaking degree, 100 mg/L concentration of antibiotic P and 0.05 mg/L concentration of gibberellin,respectively.And the content of Se in barely was 35,1.24,3.35 times higher than the controls.The research provided a theoretical basis for the producing of enriched-Se malt industrializely.