Modified cpti gene (sck) was successfully transferred into two elite inbred lines of maize E28 and 340 via particle bombardment. Fertile transgenic plants were obtained by regeneration of PPT resistant calli derived from three selections with agent PPT, and were confirmed by PCR and Southern blot assay. Expression of CpTI inhibitory activity of transgenic plants was also tested. Resistant-insect bioassay indicated that some transgenic plants showed strong resistance to corn borer.