Objective To observe the retationship of lipoperoxidation induced injury of human glomerular endothelial cells (GEC) and the membrane fluidity of GEC in high concentration glucose. Methods The culture of human GEC was established, thiobarbituric acid fluoriphotometry was used to measured MDA, Prendergast's method was used to detect the membrane fluidity of GEC, and the [Ca 2+ ]i was determined by Fura 2/AM. Results The membrane fluidity and [Ca 2+ ]i of GEC were increased significantly in high c...
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