Some factors which affect the result of Agrobacterium tumefaciens infection leaf of tea plant (Camellia sinensis) was studied based on GUS transient expression 50 mg/L Kanamycin is suitable selection concentration for expression vectors with neomycin phosphotransferase gene (NPTⅡ) as callus formation from tea plant leaf was depressed by about 30 mg/L Kanamycin The suitable system of Agrobacterium tumefaciens _mediated transformation is suggested that tea plant leaf was infected 10 min by LBA4404 ...