Several fungal strain producing lipase were screened from soil of different environment in Hefei. The optimized medium consists of (%): soybean flour 4.0, corn-steep liquor 1.0, olive oil 1.0, wheat meal 1.0, (NH 4) 2SO 4 1.0, K 2HPO 40.2, pH natural. These lipase have an optimum temperature of 24~26℃, 72h, They are stable in the pH rang of 9.4~9.5.