The plasmid pTrc harboring Glutathione synthetases gene (gsh I and gsh II) was transformed into different E. coli host strains in order to screen the best one. E. coli BL21 (pTrc gsh) can express the related enzymes with high level and stable activity. Two step culture was developed for the enzymes production. The engineering strain was incubated at 37°C and pH7.2 up to about OD 550 =0.5 during the first step. The condition was then switched to 34°C and pH6.7 after induction with IPTG(0...
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