The concentrations used for further optimization of hormones were determined by using uniform design, and then a regression formation was obtained between suspension culture Taxus chinensis cell growth and hormone's concentration in RSM one rank model. It revealed that NAA and 6-BA were the vital factors for cell growth, and the optimal result should be reached by increasing the concentrations of 2,4-D, NAA, 6-BA in the ratio of 0.23∶1∶0.62.