In this paper, we have investigated the production of γ linoleni c acid by Cunningnamella elegans. The optimum inoculum culture conditions and fermentationconditions are given as follows: inocu lating with 0.1% spore suspension, 0.2% Tween 80, naturalpH, eight layer gauz e, 30?℃,180?r/min, the culture time is 48?h; The optimum fermentation parameters are described as follows:10% inoculum, 0.1% Tween 80,pH 4.8-5.4, C/N: 33∶ 1,the appropriate termination is 168?h under 25?℃.