Objective:To compare and optimize conditions of DNA coupling to glass slides for development of DNA microarray. Methods: The process included preparation of DNA samples, spotting and post processing of arrays. DNA microarrays were prepared by spotting fluorescence labeled PCR products of target genes onto specially treated glass slides with robotics. The fluorescent signals before and after treatment were scanned with scanner, and the DNA attachment rate was calculated from the data obtained by ImaGene3.0 s...
【基金】
上海市现代生物与新药产业发展基金!资助项目( 9843 1912 1)
【分类号】
Q78
【正文快照】
基因芯片主要包括原位合成的基因芯片 [1]和直接点样的微矩阵基因芯片 ( DNA microarray) [2 ] 。其中点样于玻璃介质上的微矩阵基因芯片是目前应用最广的基因芯片 ,主要用于大规模基因表达谱的研究。微矩阵基因芯片的制备包括样品的制备与纯化、用自动点样仪进行点样、点样后?
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