Hydrophilic copper particles were prepared by using hydrazine as reductant and hydrophobic copper particles were produced in AOT (Aerosol OT,sodium bis(2 ethylhexyl)sulfosuccinate) glycerin heptane reverse micellar system. Characterized with the transmission electron microscope and the absorption spectra, the properties of the obtained nano copper particles were studied. Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix, which is compo...