A modified method for isolating of peroxisomes from rat liver was described. The peroxisome enriched fraction was put onto discontinuous sucrose density gradients and then centrifuged for 2 h at 95000×g. It was found that the peroxisomes obtained with this method were intact, and contained only minute amounts (0.5%~0.9%) cellular mitochondria and microsomes. The purity of the peroxisome was increased 26 fold compared with the liver homogenate. The final yield of peroxisomes was about 12%. The peroxisomes,...