Nine kinds of isoenzyme were analyzed by the techniques of POlyacruylamide gel electrophoresis (PAGE) among parents in tobaco. MoSt bands were constant for all varieties, but 17 bands showed intervarietal polymorphism, 2 bands ofmalic enZyme, 2 bands of glucose~6~phosphate debydrogenase, 1 band oflactate dehydogenase, 10 bands ofperoxidase, 2 bands ofesterase. The parentS were clustered into 3 groups by genetic distance based on 17 POlymol''Phic bands, most ofcultivars within same group were from same area....