Objective:To set up a method for the determination of Cholesterol in 9 kinds Cerrus for evaluating their qualities.Methods:HPLC was used in the quantitative analysis with Kromasil-C18 chromatography column(4.6 mm×250 mm,5μm)and methanol–water(93:7) as a mobile phase,at the flow rate of 1.0mL/min and the UV detection wavelength at 205 nm Results:The calibration curve of Cholesterol was linear under the content of 0.0004~0.012mg,the correlation coefficient was over 0.9993.The average recovery of Cholesterol w...