Based on the genomic sequence of NDV08-004(GenBank accession number FJ794269),seven pairs of primers were designed to amplify the genomic fragments by RT-PCR and cloned into pGEM-Teasy vector.The fragments(named A to G)were sub-cloned into transcription vector pOLTV5 according to the universal RE site and the plasmid named NDV08-004-pO which contained the full length cDNA of NDV08-004 strain was constructed.Three helper plasmids(pCI-NP,pCI-P and pCI-L)together with NDV08-004-pO were co-transfected into BSR ...
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