Avian leukosis viurs subgroup J(ALV -J) was identified in 1990's,which causes myelocytic myeloid leukosis(ML) in meat -type chicken.The envelope(env) gene of ADOL -4817 strain of ALV -J was amplified by polymerase chain reaction(PCR)and cloned into TA vector.The size ofenvgene is about 1.7kb.A transfer vector pBac4817 - envwas constructed by ligation ofenvgene DNA and pBlue - Bac4 plasmid DNA.By cotransfecting Sf9 cells with both Bac - N - Blue baculovirus DNA and pBac4817 - envDNA,a recombinant baculovirus...
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