To construct a cDNA library of mature leaves( Polygonum multiflorum Thunb.),so as to further research the gene regulation of secondary metabolite biosynthesis of offcinal.Total RNA was extracted with improved CTAB method and mRNA was purified by oligo(dT)-cellulose column chromatography,which were synthesized to double strands through reverse transcription。After blunting the cDNA termini,the 5’end of EcoR I adapters phosphorylated were conjoined and then digested by Xho I。cDNA fragments less than 400bp were...
【英文论文摘要】
To construct a cDNA library of mature leaves( Polygonum multiflorum Thunb.),so as to further research the gene regulation of secondary metabolite biosynthesis of offcinal.Total RNA was extracted with improved CTAB method and mRNA was purified by oligo(dT)-cellulose column chromatography,which were synthesized to double strands through reverse transcription。After blunting the cDNA termini,the 5’end of EcoR I adapters phosphorylated were conjoined and then digested by Xho I。cDNA fragments less than 400bp were...
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