This paper studied several major factors influencing the regeneration of‘Toyonoka' strawberry in the anther culture such as low-temperature pretreatment time,the optimal temperature of inducing callus,the best hormones combination,the callus types of influencing bud differentiation and so on.The results showed that the young flower treated for 3days at 4℃and the anther cultured at 20℃were useful.Yellow-green granular callus was best to regenerate in the anther culture.Medium supplied with 0.2mg·L~(-1) BA,1....