茶叶绿色荧光物质是一类在490nm光激发下能发射515nm绿色荧光的未知化合物,为了对其进行结构鉴定,本文通过研究高效液相色谱的色谱柱类型、流动相组成及洗脱梯度等色谱条件,旨在建立适用于茶叶绿色荧光组分结构鉴定的HPLC分析方法。结果表明:⑴适宜的HPLC色谱条件为:极性封端的Hypeisil GOLD aQ C18色谱柱;流动相由A相10mmol-L-1乙酸铵水溶液(pH=7.0)和B相甲醇组成;线性梯度洗脱程序为0→5min,0%B相,5→65min,0%→60%B相;流速0.8mL/min;柱温28℃;进样量10μL,以上色谱分离条件可满足茶叶绿色荧光物质的荧光检测、紫外-可见光谱和质谱分析的要求;⑵应用建立的HPLC方法分离检测到5种主要绿色荧光组分,且各色谱峰均可达基线分离,保留时间合理。
【英文论文摘要】
Green fluorescence substances in tea leaves (TGFS for short) are a type of unknown compounds which can emit green fluorescence (maximum wavelength at 515nm) by excited at 490nm. This article was aimed to establish an HPLC analysis method suitable to identify the structure of TGFS, and then optimized HPLC conditions such as types of chromatographic columns, composition of mobile phases, elution gradient etc. Results showed that: ⑴ suitable HPLC conditions were as follows: Hypersil GOLD aQ C18 column was used...
| 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
