GPMV NA-1 was multiplied by SPF embryonated eggs and purified,and then to extract the RNA genome of virus. Based on the complete genome sequence of goose paramyxovirus strain NA-1 logged in GenBank and then two pairs specific primers were designed to amplified M,F and HN genes fragment by the method of RT-PCR.Meanwhile,the target gene fragments were respectively cloned into the pET-28a and were dentificated by restriction enzyme digestion and sequencing analysis.The positive recombinant plasmids were named ...
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