Effects of different cultivation patterns and substrates on growth and development of Paeonia lactiflora ' Oiao Ling' was studied in this paper.The results indicated that the growth and development of 'Qiao Ling' grown in the field was better than potted plant.Plants grew differently in different substrates.Under the mixed substrate turf+vermiculite+perlite 3:1:1, the growth and blooming of plants was best.