The antifreeze effect of carrot antifreeze protein (DcAFP) prepared by gene engineering on E.coli and rice cells treated by cryopreservation was studied.The results showed that gradient concentration DcAFP can distinctly improve survival rate (similar to positive control) of bacteria treated with three temperatures.However,DcAFP displays higher capability of protecting rice cells than positive control during the three temperature cryopreservation,moreover,the cell viability and relative viability derived fr...