Microsatellite enrichment by magnetic beads,fast isolation by the AFLP of sequences containing repeats (FIASCO protocol),was used to isolate and characterize microsatellite molecular markers of Nelumbo nucifra Gaertn.by Biotin-(AG)13 as probe.42 positive clones were sequenced.36 of the sequenced clones(about 85 %)contained SSR lo- cus,among which 63.9 %(23 clones)microsatellites repeated more than 10 times and 52.8 %(19 clones)microsatellites were perfect repeats.Then,24 pairs of primers were designed,and 1...