Callus induction and plant regeneration should always be vital process to create transgenic plants. Compared with dicotyledonous plants, researches of callus induction and plants regeneration of cereal plants, especially wheat, is relative slow. In order to give some effective information for wheat callus induction, the callus induction of Longjian 127 and Shan 160 mature embryos was carried out with quadratic orthogonal rotation combination designs in vitro culture of revised Murashige and Skoog recipe. Th...