γ-aminobutyric acid in tea was extracted, isolated and determined. Water achieve a better effect than ethanol in extracting γ-aminobutyric acid, and γ-aminobutyric acid content was enhanced by 20%; 732 positive ion resin columns (2.5×50cm) was used to isolate, citric acid and ammonia water were used as elutes, based on pH linear gradient. Results indicated that γ-aminobutyric acid inelute could reach 39%; thin layer scanner could measure the content of γ-aminobutyric acid.
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