Purpose To establish the method for extracting the protease from the alimentary canal of pneumatophorus japonicus,and were studied on its optimum extraction conditions. Methods The protease was performed by ethanol extract.Results The extraction temperature of the protease was 20℃, the extraction time was 2 hours, pH was 7.5,stimulation time was 16 hours,the concentrations of ethanol precipitation was 65%.Conclusion The method might be simple,accurate and suitable for the extraction of the protease.
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