Objective Human nerve growth factor is the biologically active growth factor that plays an important role in the human body regulation. NGF has been the extremely useful medicine in clinic while the natural hNGF is difficult to obtain. The study is to prepare the engineering hNGF. Methods We constructed the efficient expression plasmid and transformed it into Pichia pastoris, which was applied for screening and expression. Results The engineering strain with high-level expression and secretion was obtained....
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