利用4个水稻品种培矮64S、9311、广陆矮4号和日本晴(Nipponbare)进行杂交组合,获得6个 F_2群体(PA64S/9311、 PA64S/Nipponbare、PA64S/GLA、9311/GLA、Nipponbare/GLA 和 Nipponbare/9311)。除 PA64S/Nipponbare 群体为180个单株外,其余5个群体均为90个单株。在成功开发出新 SSR 标记的基础上,合成756对 SSR 引物对4个亲本进行了多态性检测,并随机选用一些具有多态性的标记对 F_2群体进行了多态性分析,采用 MAPMAKER/EXP 3.0软件构建了6张微卫星连锁图谱。覆盖全基因组的长度为825.6~2455.2 cM,定位的标记位点数为54~152个,标记间的平均遗传距离为11.81~16.15 cM。比较分析结果表明,6张图谱间及与 Ternnykh 等绘制的图谱在标记的染色体分布和线性排列上具有较高的一致性。选用测序品种作为构图亲本有助于解析水稻基因组遗传信息,而开展水稻亚种间比较作图研究则可以促进整个禾本科植物比较基因组研究的发展。
【英文论文摘要】
Six F_2 populations were derived from four sequenced rice varieties between PA64S,9311,Nipponbare and GLA,they were PA64S/9311 F_2,PA64S/Nipponbare F_2,PA64S/GLA F_2,Nipponhare/GLA F_2,Nipponhare/9311 F_2 and 9311/GLA F_2.Each F_2 population had 90 lines except for PA64S/Nipponbare(180 lines).Based on the successful development of rice new microsatellite mark- ers,756 pairs of simple sequence repeats(SSRs)primers were synthesized in this research to analyze their polymorphisms between the four parents,then ...