The types of DNA molecular markers include RFLP, RAPD, DAF, SPAR, SSR, SCAR, STS, AFLP, CAPS, SNP, and so on. The major application of DNA molecular markers in maize genetic breeding are prediction heterosis and classification of het-erotic groups; mapping genes and master assisted selection; costruction of the frame molecular linkage map; DUS testing and purity testing for hybrids. As a new method for research, DNA molecular markers will create more wide applied foreground in the field of maize breeding.