Variety purity test is the requisite for variety protection and seed production of maize, which plays great role in maize production. In this paper, methods and techniques of maize variety purity test was reviewed. The accuracy of morphological evaluation method was not up to standard due to its great response to environmental factors and lack of effective traits needed. Isozyme and seed storage protein were insufficient number of polymorphic loci, which could not differentiate the varieties with close pedi...