The wheat disease samples with typical sharp eyespot from Zhoukou, Anyang,Nanyang etc13 regions in Henan Province were obtained,and then 157 Rhizoctonia strains were isolated.All of the isolates were binucleate Rhizoctonia strains (BNR) identified by the nuclear staining,while 156 isolates belonged to the AG-D anastomosis group,1 was the AG-BO anastomosis group by hyphal anastomosis reaction and rDNA-ITS sequences analysis.The virulence of 113 isolates were tested in greenhouse on 3 wheat cultivars such as ...