Construction of molecular genetic map and agronomic trait would be useful for spreading peat genus resources and marker-assisted selection for genetic improvement in pear. In this study, SSR primers were developed based on the selected SSR sequences from the EST sequences of pear in GenBank and GDR database of NCBI, and used for analysing genetic diversity within F1 progeny of Pyrys pyrifolia Nakai 'Xizilv×Kisui'. SSR, EST-SSR, RAPD technology which were used to analyze pear genome DNA were established firs...