Salt stress seriously affected the growth and development of plant, while the vacuolar membrane Na+/H+ antiporter could transport and compartment the excess Na+ into vacuoles, playing an important role in plants for salt tolerance.
The cDNA of NHX1 gene which was then designated as NnNHXl was successfully isolated from 'Donghe'by RT-PCR and RACE cloning method. The full-length cDNA of NnNHX1 was 2237 bp, included a 426 bp 5'UTR, a 194 bp 3'UTR, and a 1617 bp ORF which was predicted to encode a 59.12 kDa...