Harpin蛋白是由革兰氏阴性植物病原细菌Hrp(hrp,hypersensitive response and pathogenity)基因簇中hrp基因编码的、性质和功能相似的一类蛋白质,富含甘氨酸,缺少半胱氨酸,对蛋白酶敏感,对热稳定,大多对寄主植物具有毒性,导致寄主发病,能在非寄主植物上引起过敏反应(hypersensitive response,HR)增强植物抗旱性,诱导植物抗病、抗虫,还可以促进植物生长。
popW是本实验室从青枯劳尔氏菌(Ralstonia solanacearum) ZJ3721中鉴定出的编码一种新的harpin蛋白大小为1142bp的基因,其编码的PopW蛋白是一种新的harpin蛋白,它同以前报道的harpin类物质具有相同的性质:酸性,富含甘氨酸和丝氨酸,缺少半胱氨酸并且是一种耐热蛋白。通过对PopW蛋白原核表达条件的优化发现:随着IPTG浓度的增加,诱导表达的蛋白含量也会随之增加,当IPTG的浓度为0.03 mM,诱导表达的蛋白含量最高,若继续增加IPTG的浓度,蛋白的表达量反而下降。但鉴于成本因素,而且0.01 mM的诱导效果与0.03mM并无显著差异,因此在...
【英文摘要】
Harpins are extra cellular proteins encoded by Gram-negative bacterial hrp (hypersensitive response and pathogenity) gene cluster. They are a major class of proteins that travel the pathway, elicit the HR (hypersensitive reaction) when infiltrated into the apoplast of leaf tissue (hypersensitive cell death.HCD) drought tolerance, defense responses against pathogens and insects in plants, and they can enhance Plant growth as well. They are heat stable, rich in Gly and/or Ser, lack Cys, and differ in their pr...