Special molecular markers were developed for Betula luminifera using Birch ESTs (Expression sequence tags) data. We then used these markers to evaluate the genetic diversity of B.luminifera germplasm. Based on their genetic distance, B.luminifera core germplasm was constructed. The main conclusions were as follows:
1.Based on birch EST data, primers for 109 ACGM candidate loci were developed and tested in birch. Of these, 105 yielded single, stable and clear PCR products. We then tested the utility of t...