沙蒿多糖(Artemisia sphaerocephala polysaccharide,ASP)是从沙蒿籽榨油后剩余的废渣中提取的多糖类物质,其主要成分为半乳甘露聚糖,是高度分支的线形大分子,具有良好的降血糖作用和一定的抗氧化活性。沙蒿多糖硫酸化衍生物(Sulfated derivatives of ASP,SASP)、磷酸化衍生物(Phosphated derivatives of ASP,PASP)和硒酸化衍生物(Selenide of ASP,SeASP)分别是以ASP为原料,通过化学反应法对其结构进行硫酸化、磷酸化和硒酸化修饰而制得,前人研究发现其体外抗氧化活性得到一定的提高。因此,本实验采用肝癌HepG2细胞研究ASP、SASP、PASP、SeASP的体外降血糖活性差异:通过观察ASP、SASP、PASP、SeASP对HepG2细胞生长曲线的影响,探讨ASP、SASP、PASP、SeASP对细胞生长期的作用特点;并通过体外葡萄糖消耗实验和四甲基偶氮哇蓝(MTT)毒性实验来初步探索ASP、SASP、PASP、SeASP对HePG2细胞的降血糖作用和毒性效应;优化胰岛素的诱导条件,建立稳定的HepG2...
【英文摘要】
Artemisia sphaerocephala polysaccharide(ASP) is a polysaccharose substance from waste residue when Artemisia sphaerocephala Krasch. seeds are oil pressed, essential constituent of which is galactomannan, a linear macromolecule with many branched chains and it has been studied for well hypoglycemic effect and antioxidant activity. Sulfated derivatives of ASP(SASP), Phosphated derivatives of ASP(PASP) and Selenide of ASP(SeASP) are prepared using ASP as raw materials by chemical structure modified of sulfatio...
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