Radial flow chromatography (RFC) was a new technique of chromatography purification. A special radial flow design was applied in RFC. Compared with axial flow, RFC has several prominent advantages like higher flow rate, larger throughput, lower column pressure and easier linear amplication. In this study, radial flow column was used for the purification of crude polysaccharides from Ganoderma lucidum. The main work and results were as follows.
1. The results of single factor experiments showed: ion-exch...