In this thesis, a lipase-producing strains was screened and the enzyme activity of lipase was researched. The strain of lipase-producing was made as microbial additives suspension and the mice were fed by it. The effect of growth, immune function, and intestinal microbial communities of mice were detected.Then the gene of lipase was cloned and expressed. The results were showed as follow:
1.8 lipase-producing strains was screened from soil which stained by oil. Through enrichment, plate isolation, flask...