本文以胀果甘草(Glycyrrhiza inflata Bat.)内生菌为研究对象,用非培养法研究了甘草根内生细菌的生物多样性,为开发新的微生物资源奠定基础。
采集新疆库尔勒紫泥泉甘草自然保护区的甘草根,经严格表面灭菌,以改进的CTAB(十六烷基三甲基溴化铵)法提取新疆野生胀果甘草根部总DNA基因组,利用细菌16S rDNA基因通用引物799f-1492r对甘草总DNA进行16S rDNA基因扩增,构建甘草根内生细菌16S rDNA克隆文库,获得阳性克隆150个,将经过ARDRA (Amplifying rDNA Restriction Analysis)分型的克隆进行测序,得到32个OUT,与由美国国立生物技术信息中心(National Center for Biotechnology Information简称NCBI)建立的GenBank序列数据库中的序列进行比对,找出相似性最高的序列,并用MEGA3.1构建16S rDNA基因系统发育树。
Blast结果表明大部分克隆(74%)与已知细菌的16S rDNA基因序列相似性较高,分别与变形杆菌门(Proteobacteria)的alpha、...
【英文摘要】
We investigated endophytic bacterial diversity in root of Glycyrrhiza inflata Bat. from Xingjian by culture-independent method,aimed to lay a foundation for furthering to tap.
Glycyrrhiza roots were sampled from Ziniquan, Kuerle, in XinJiang province, the original habitat. Samples were strictly surface-sterilized followed by CTAB (Hexadecyl trimethyl ammonium Bromide) procedure with some modifications. A pair of bacterial PCR primers was used for endophytic bacterial 16S rDNA gene amplification and a cl...