Recently, sensitive detection of disease-related proteins is critical to many areas such as modern biomedical research and clinical diagnosis. Immunoassay is one of the most important analytical techniques in the quantitative detection of disease markers with selectivity due to the highly specific molecular recognition between antigen and antibody. In comparison with other immunological methods such as radioimmunoassay, fluorescence immunoassay and chemiluminescence immunoassay, electrochemiluminescence imm...